Green Genes
Clone a gene and make a fluorescent protein
Green Genes is offered in-person at all DNALC locations.
In-Person Green Genes
Green Genes is a challenging summer camp designed to apply and build on knowledge gained in Fun with DNA and World of Enzymes. In this biotechnology camp, students learn more about the practical applications of recombinant DNA technology.
Through a series of lab experiments, students utilize many of the same techniques employed by pharmaceutical companies to produce human insulin. Through cloning and expressing the Green Fluorescent Protein (GFP) gene, normally found in the Pacific jellyfish Aequoria victoria, students work each day towards a final goal of extracting and purifying a jellyfish protein from genetically engineered bacteria. Each experiment acts as a stepping stone for the next day's work, requiring students to demonstrate proper lab techniques. Students will:
- use enzymes to cut and paste genes to form a functional plasmid;
- analyze results with gel electrophoresis;
- genetically engineer bacteria to produce a visible protein;
- use polymerase chain reaction to amplify DNA fragments; and
- isolate and purify GFP using chromatography.
Details:
- Grades: entering grade 9 or World of Enzymes alumni entering grade 8
- $600 per student
- Monday to Thursday 9:30 a.m.–2:30 p.m., Parent Participation Day Friday, 11:30 a.m.–1:00 p.m. in CSH and 9:30 a.m.–11:00 a.m. in NYC, Sleepy Hollow, and NJ
- June 29–July 3 week (at all locations except LI) is Monday–Thursday due to the Independence Day holiday. Parent Participation Day will be Thursday 2:30 p.m.–4:00 p.m. with no class Friday.
- June 29–July 3 week at Long Island location is Monday–Thursday 9:00 a.m.–2:00 p.m., Parent Participation Day Thursday 1:30 p.m.–2:30 p.m.
- Available at:
