Green Genes

In-Person Green Genes

Green Genes is a challenging summer camp designed to apply the knowledge gained in Fun with DNA and World of Enzymes. In this biotechnology camp, students learn more about the practical applications of recombinant DNA technology.

Through a series of lab experiments, students utilize many of the same techniques employed by pharmaceutical companies to produce human insulin. Through cloning and expressing the Green Fluorescent Protein (GFP) gene, normally found in the Pacific jellyfish Aequoria victoria, students work each day towards a final goal of extracting and purifying a jellyfish protein from genetically engineered bacteria. Each experiment acts as a stepping stone for the next day's work, requiring students to demonstrate proper lab techniques. Students will:

• use enzymes to cut and paste genes to form a functional plasmid;
• analyze results with gel electrophoresis;
• genetically engineer bacteria to produce a visible protein;
• use polymerase chain reaction to amplify DNA fragments; and
• isolate and purify GFP using chromatography.